Semaglutide intermediate Fmoc-Ala-Ala-OH CAS 87512-31-0 HPLC 99+

Product Description

Semaglutide intermediate Fmoc-Ala-Ala-OH CAS 87512-31-0 HPLC 99+

Name: Fmoc-Ala-Ala-OH

CAS NO: 87512-31-0

M.W:382.41

Appearance: White Powder

Purity: 98+   

Synonyms: Fmoc-L-Alanyl-L-Alanine

Application

To synthesize semaglutide using Fmoc-Ala-Ala-OH as a potential intermediate, the general steps involve the following:

Design the Peptide Sequence: First, determine the complete amino acid sequence of semaglutide.

Solid-Phase Peptide Synthesis (SPPS):

Resin Loading: Start the peptide chain synthesis using a resin (such as polystyrene resin) as the solid-phase support.

Stepwise Coupling: Gradually couple Fmoc-protected amino acids (including Fmoc-Ala-Ala-OH) or other fragments to the resin, following the amino acid sequence of semaglutide. This involves removing the N-terminal Fmoc protecting group to expose the free amino group, followed by condensation with the carboxyl group of the next amino acid.

Removing Protective Groups: During the synthesis, certain amino acid side chains may require protection to avoid unwanted reactions. These protecting groups need to be removed at the appropriate time.

Washing and Drying: After each step, wash the resin with appropriate solvents (such as DMF, DCM, methanol, etc.) to remove unreacted reagents and byproducts, and then dry it.

Release of the Peptide Chain: Once the peptide chain synthesis is complete, it needs to be released from the resin. This is typically achieved by cleavage using a strong acid, such as trifluoroacetic acid (TFA).

Purification: The released peptide chain often contains impurities and requires purification using chromatography methods such as high-performance liquid chromatography (HPLC).

Characterization: Characterize the purified peptide using techniques such as mass spectrometry (MS) and/or nuclear magnetic resonance (NMR) to ensure its correct sequence and structure.

Activity Assessment: If possible, evaluate the biological activity of the synthesized semaglutide to ensure it meets the expected pharmacological effects.

Package

Transportation

Small package(1g, 25g, 1Kg, 25Kg) can be shipped by Express. (DHL, FedEx, EMS, etc.)
Large package(100kg and more than100Kg) can be transported by Air or Sea.

All transportation is in accordance with the customer's needs.

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